Stem cell update
Chromatin signatures of pluripotent cell lines.Azuara V, Perry P, Sauer S, Spivakov M, Jorgensen HF, John RM, Gouti M, Casanova M, Warnes G, Merkenschlager M, Fisher AG. Nat Cell Biol. 2006 Mar 29; [Epub ahead of print]
Enhanced purification of fetal liver hematopoietic stem cells using SLAM family receptors. Kim I, He S, Yilmaz OH, Kiel MJ, Morrison SJ. Blood. 2006 Mar 28; [Epub ahead of print]
As low as 1 HSC of fetal liver was transplanted. One out of 7.7 transplants (13%), CD48-Sca1+Lin-Mac1+ (SLM) fetal live cells gave rise to long-term multilineage reconstitution in irradiated mice, while 1 out of every 8.9 (11%) CD48-Thy(low)Sca-1(+)lineage-Mac-1(+) fetal liver cells do so. With addition of CD150, CD150+CD48-SLM cells give rise to long-term multilineage reconstitution at frequency of 30-42%. Fetal liver HSC were also found in CD41- and CD244- population. SLAM family receptors CD150, CD244, and CD48 are therefore shown enrichment markers for fetal liver HSC. The authors also emphasized the role of SLAM family receptors in distinguishing "progenitors at different stages of the hematopoiesis hierarchy and enhance the purification of definitive HSCs from diverse contexts".
Interestingly, they were not detectably expressed by stem cells in the fetal or adult nervous system.
Reactive oxygen species act through p38 MAPK to limit the lifespan of hematopoietic stem cells. Ito K, Hirao A, Arai F, Takubo K, Matsuoka S, Miyamoto K, Ohmura M, Naka K, Hosokawa K, Ikeda Y, Suda T. Nat Med. 2006 Mar 26; [Epub ahead of print]
In vitro germline potential of stem cells derived from fetal porcine skin. Dyce PW, Wen L, Li J. Nat Cell Biol. 2006 Mar 26; [Epub ahead of print]
Enhanced purification of fetal liver hematopoietic stem cells using SLAM family receptors. Kim I, He S, Yilmaz OH, Kiel MJ, Morrison SJ. Blood. 2006 Mar 28; [Epub ahead of print]
As low as 1 HSC of fetal liver was transplanted. One out of 7.7 transplants (13%), CD48-Sca1+Lin-Mac1+ (SLM) fetal live cells gave rise to long-term multilineage reconstitution in irradiated mice, while 1 out of every 8.9 (11%) CD48-Thy(low)Sca-1(+)lineage-Mac-1(+) fetal liver cells do so. With addition of CD150, CD150+CD48-SLM cells give rise to long-term multilineage reconstitution at frequency of 30-42%. Fetal liver HSC were also found in CD41- and CD244- population. SLAM family receptors CD150, CD244, and CD48 are therefore shown enrichment markers for fetal liver HSC. The authors also emphasized the role of SLAM family receptors in distinguishing "progenitors at different stages of the hematopoiesis hierarchy and enhance the purification of definitive HSCs from diverse contexts".
Interestingly, they were not detectably expressed by stem cells in the fetal or adult nervous system.
Reactive oxygen species act through p38 MAPK to limit the lifespan of hematopoietic stem cells. Ito K, Hirao A, Arai F, Takubo K, Matsuoka S, Miyamoto K, Ohmura M, Naka K, Hosokawa K, Ikeda Y, Suda T. Nat Med. 2006 Mar 26; [Epub ahead of print]
In vitro germline potential of stem cells derived from fetal porcine skin. Dyce PW, Wen L, Li J. Nat Cell Biol. 2006 Mar 26; [Epub ahead of print]
posted by Zhang at 7:53 PM
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